Greater severity of meconium stained amniotic fluid associated with increased maternal infectious morbidity: Study
Meconium-stained amniotic fuid (MSAF) is observed in 5% to
20% of laboring patients, with the incidence reaching approximately 27% in
post-term pregnancies. The presence of meconium in the amniotic fluid has been
linked to fetal acidemia, hypoxia, and hypoxic-ischemic encephalopathy, meconium
aspiration syndrome, seizures, and neonatal mortality. The relationship between
MSAF and clinical chorioamnionitis, as well as neonatal sepsis, has been
documented, yet the precise mechanisms remain unclear. Additionally, the
correlation between prolonged labor and MSAF was described in the past.
In women with prolonged rupture of membranes (ROM) and
intrapartum fever (IPF), MSAF increased the risk of isolating
Enterobacteriaceae from chorioamnionitic swabs. In a study examining the impact
of MSAF on bacterial growth in amniotic fluid, it was observed that even low
concentrations of meconium (up to 1.5 mg/ml) inhibited the growth of
Escherichia coli. In light of these conflicting findings, a study aimed to
explore the relationship between the thickness of MSAF and the risk of maternal
infectious morbidity. Additionally, authors aimed to assess the distribution of
pathogens in chorioamnionitic swab cultures relative to the varying degrees of
MSAF thickness.
A retrospective study was carried out of 15,950 term
singleton pregnancies at a tertiary hospital (2020–2024). Women were
categorized into four groups based on the presence and thickness of MSAF:
clear, light, intermediate, and thick. The co-primary outcomes were clinical
chorioamnionitis and puerperal endometritis, defined as a composite maternal
infectious morbidity. In women with intrapartum fever (IPF), chorioamniotic
swabs were obtained and compared according MSAF thickness. Multivariate
analysis identified predictors of a composite maternal infections and adverse
neonatal outcomes.
Of the cohort, 13,745 had clear amniotic fluid, and 2,205
had MSAF (561 light, 1,426 intermediate, 218 thick). The incidence of maternal
infections increased with MSAF thickness, with thick MSAF showing the highest
rates of clinical chorioamnionitis (4.1%, p< 0.001) and endometritis (1.4%,
p=0.039). In IPF cases, thicker MSAF was associated with a higher prevalence of
positive swab cultures, particularly of Enterobacteriaceae (61.9%). Group B
Streptococcus (GBS) remained consistent across all MSAF groups. Multivariate
analysis showed that MSAF levels were associated with increased maternal
infectious morbidity (p< 0.001). Additional risk factors for maternal
infections included nulliparity (p< 0.001), catheter balloon insertion
(p=0.004), prolonged ROM (p< 0.001), and cesarean delivery (p< 0.001). In
contrast, only intermediate (p< 0.001) and thick MSAF (p< 0.001)
correlated with adverse neonatal outcomes.
The rates of clinical chorioamnionitis and puerperal
endometritis were observed to increase proportionally with the severity of
MSAF. In women with intrapartum fever, thicker MSAF was associated with a
higher prevalence of Enterobacteriaceae in chorioamnionitic swabs, though GBS
isolation rates remained consistent. Multivariate analysis demonstrated that
all MSAF degrees were associated with an increased risk of maternal infectious
morbidity. Additional risk factors for maternal infections identified in the
analysis included nulliparity, catheter balloon insertion, prolonged ROM
duration, and CD. In contrast, only intermediate and thick MSAF correlated with
adverse neonatal outcomes, while light MSAF did not show the same association.
Increasing severity of MSAF was associated with maternal
infectious morbidity and particularly Enterobacteriaceae related infections.
This underlines the importance of close monitoring and the potential need for
preventive measures in cases where thick MSAF is present. Studies about
preventive measures in cases of thick MSAF are warranted.
Source: Raneen Abu Shqara, Lior Lowenstein,
Maya Frank Wolf; Archives of Gynecology and Obstetrics https://doi.org/10.1007/s00404-024-07808-4